Supplementary Figure 2: Characterization of EVs. | Nature Cell Biology

Supplementary Figure 2: Characterization of EVs.

From: Cancer-cell-secreted exosomal miR-105 promotes tumour growth through the MYC-dependent metabolic reprogramming of stromal cells

Supplementary Figure 2

(a) EVs pelleted at 110,000 ×g were analysed by nanoparticle tracking analysis (n = 3 independent experiments). The black line indicates mean, whereas the red shaded area indicates SEM. (b) Numbers of secreted EVs determined by nanoparticle tracking analysis (n = 3 independent experiments). (c) A schematic representation of the gradient separation procedure to further characterize the 110,000 ×g pellets. (d) Western blot, density measurement, and RT-qPCR of fractions collected from gradient separation indicating miR-105 enrichment in fractions containing EV markers (n = 3 independent experiments). (e) RT-qPCR-determined levels of miR-105 and miR-155 in equal amounts of EVs from indicated cells (normalized to cel-miR-39-3p spike-in control) and in cell extracts (normalized to U6) (one-way ANOVA, n = 3 independent experiments). For the entire figure, data are shown as mean ± SD; **P < 0.01, ***P < 0.001. †undetectable. Unprocessed original scans of blots are shown in Supplementary Figure 9. Source data are shown in Supplementary Table 5.

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