Supplementary Figure 6: Analysis of transcripts deregulated by non-degradable roquin2(Y691F) mutant.

a, Gene ontology (GO) analysis of genes regulated by the non-degradable roquin2(Y691F) mutant. Bar plot for the −log₁₀ of the P value of the top 10 enriched GO terms of genes regulated by roquin2(Y691F) as determined by the hypergeometric distribution. −reg., negative regulation; +reg., positive regulation. Statistical analysis and genes list are provided in Supplementary Table 4. b, Ranking of roquin2-regulated genes by the percentage of genetic alteration in human DLBCLs from the TCGA database along with the base mean expression from the RNA-seq analysis. The cut-off was set at 6%. 'Yes' indicates transcripts that are dependent on the ROQ domain of roquin2; 'No' indicates transcripts that are not dependent. (c) Level of indicated mRNAs analysed by quantitative PCR from U2932 cells stably expressing HA–roquin2(WT), HA–roquin2(Y691F) or HA–roquin2(Y691FΔROQ) and treated with 10 μg ml⁻¹ of F(ab′)₂–IgM for 12 h. The value for each PCR product present in HA–roquin2(WT) cells was set as 100%. Rescued transcripts are defined as ones whose levels reach at least 70% of the roquin2(WT) control (indicated by the dashed line) (mean ± s.d., n = 3 independent experiments). Source data for c can be found in Supplementary Table 6.