Supplementary Figure 2: Dynamics of H3K9me3 during mouse early embryo development.

a, Boxplots show the H3K9me3, H3K27me3 and DNA methylation level of Oocyte specific, Cleavage specific and Blastocyst specific H3K9me3 domains. Statistical significance between stages was evaluated based on one-sided Wilcoxon test, * represent p-value < 0.05, *** represent p-value < 0.001. Oocyte specific (n = 38996 H3K9me3 domains), Cleavage specific (n = 114033 H3K9me3 domains), Blastocyst specific (n = 119837 H3K9me3 domains). The center represents the median value and the upper/lower line represent 5% and 95% quantile for the boxplots. b, Heatmaps show the dynamic of H3K9me3, H3K27me3 and DNA methylation level in blastocysts and post-implanted embryos. The heatmaps were generated using the same order of pre-implantation H3K9me3 heatmap. Colors represent log2-transformed ChIP / input ratio scaled by row, or absolute DNA methylation level. ChIP-seq for H3K9me3 has been performed twice for each sample and data shown represent averages for these independent experiments. H3K27me3 ChIP seq data for zygote was performed three times and data for pre-implantation embryos were from our previous publication (GSE73952). WGBS has been performed once for each sample.