Supplementary Figure 4: Overexpression of PAX5 and PRDM1 enhances germ cell differentiation of hESCs in vivo in xenotransplantation.
From: A PAX5–OCT4–PRDM1 developmental switch specifies human primordial germ cells
(a) Immunostaining of GFP and DAPI in untransplanted mouse testis. Scale bars represent 100 µm. Immunostaining experiments were independently repeated a minimum of three times with similar results. (b) Immunostaining analysis of testis xenografts derived from PAX5&PRDM1 double OE H1 hESCs. All images are merged from DDX4 (red), GFP (green) and DAPI-stained nuclei. Scale bars represent 50 µm. Immunostaining experiments were independently repeated a minimum of three times with similar results. (c) Percentage of tubules positive for GFP+/ DDX4+ cells was calculated across multiple cross-sections (relative to total number of tubules). Data are represented as mean ± SD of n=3 independent replicates. (d) For each positive tubule, the ratio of GFP+/DDX4+ cells per tubule was determined. Data are represented as mean ± SD of n=3 independent replicates. Source data for c and d are in Supplementary Table 2.
