Supplementary Figure 2: PAX5 and PRDM1 expression and binding in hPGCs.
From: A PAX5–OCT4–PRDM1 developmental switch specifies human primordial germ cells
(a) Cross-section of human fetal testis (22 weeks) with immunostaining for OCT4, PAX5, PRDM1, SOX2 and IgG control. Scale bars represent 50 µm. Immunostaining experiments were independently repeated a minimum of three times with similar results. (b) Expression level of transcription factors in hESCs and human fetal testis. Data are represented as mean ± SD of n=3 independent replicates. (c) Heatmap visualization of PAX5 and PRDM1 ChIP-seq data, depicting all binding events centered on the peak region within a 5kb window around the peak. (d) GST-pull down assay to assess protein interactions between PRDM1 and PAX5. Western blot images are representative of three independent experiments with similar results. Unprocessed scans of western blot analysis are available in Supplementary Fig. 8. Source data for b are in Supplementary Table 2.
