Supplementary Figure 6: Properties of single-49f CB cell RNA-seq libraries.

(a) Percent of reads for each single-cell library mapped to ribosomal (left), mitochondrial (middle), or spike-in control RNA (right). Median values are shown as dashed lines. Library size and number of expressed genes per cell are shown in (b) and (c), respectively (n= 432 cells from 2 separate donors). Median values are shown as dashed lines. (d) Distribution of gene expression values (n= 58,143). The dashed blue line indicates the minimum threshold for inclusion. (e) Library size by computed size factor for data normalization (n=411 cells from 2 separate donors). (f) Gene dispersion as a function of average expression (n=411 cells from 2 separate donors). Genes included for PCA upstream of t-SNE are labelled.