Supplementary Figure 7: Characteristics of single-49f CB cell PBAL sequencing libraries.

(a) Distribution of CpG coverage for negative controls (n=6), single cells (n=140), and 10-cell controls (n=12). Alignment rate per cell is indicated by colour. For all box plots, the box shows the interquartile range, and whiskers extend to the furthest data point no more than 1.5x the total box length from the edge of the box. (b) Measured global methylation levels per cell for the CD33⁺CD90⁺ subset of 49f CB cells, and all other 49f CB cells (n=136 single cells, 12 ten-cell controls, 2 separate CB donors). Conversion rates for single cells and 10-cell controls are shown for the positive (Lambda DNA) and negative (T7 DNA) controls in (c) and (d), respectively (n=140 single cells, 12 ten-cell controls, 2 separate donors). Boxes-and-whisker plots are as in (a). (e) CNV in autosomal chromosomes (n=140 single cells, 2 separate donors). The threshold for calling a cell abnormal is shown as a solid line. (f) Number of genes associated with each DMR that was hypomethylated in the CD33⁺CD90⁺ subset (left, n=11,498 regions) or any other 49f CB cell (right, n=15,926 regions). (g) Distance to the associated transcriptional start sites (TSS) for DMRs associated with genes determined using rGREAT (n=4,725 and 5,925 regions for CD33⁺90⁺ and other 49f CB cells, respectively)