Supplementary Figure 1: Data normalization for cross-platform comparison of flow and mass cytometric data.

(a) Gating hierarchy for cells in the CD49f CB population using mass cytometry. (b) Raw data (left) from 3 independent index sort FACS experiments are shown in blue, magenta and aquamarine, and from mass cytometry experiments in grey. Rank-normalized data (used for determining nearest-neighbours for sorted cells in the mass cytometry data) are shown in the middle panels. The right panels show mean-scaled data (channel z-score) for the flow cytometry data only (used in surface marker comparisons between experiments).