Supplementary Figure 5: Single cell characterization of hematopoietic progenitors stimulated with cytokines. | Nature Cell Biology

Supplementary Figure 5: Single cell characterization of hematopoietic progenitors stimulated with cytokines.

From: Single-cell characterization of haematopoietic progenitors and their trajectories in homeostasis and perturbed haematopoiesis

Supplementary Figure 5

a, Experimental procedure of cytokine stimulation. Mice were injected with erythropoietin (Epo) or granulocyte-colony stimulating factor (G-CSF) for two consecutive days. Mice were sacrificed on third day (48 hours) and tiers 3 & 7 single cells were collected and processed for MARS-seq. b-c, Fold change in bone marrow composition (b) and Tier 3 (c-Kit+, c) fraction in the bone marrow of cytokine treated mice as determined by FACS. (Supplementary Table 5). d, Frequencies of transcriptionally annotated lineages (as in Fig. 2) in Epo or G-CSF treated mice vs. PBS treated control. Horizontal lines indicate mean. e, Distribution of the stem-score in tier 7 cells (CD150+ LT-HSC) collected from Epo treated, G-CSF treated and untreated mice. The central mark in box plot is median, with 5/95 percentiles at the whiskers and 25/75 percentiles at the box. Two sided Kolmogorov-Smirnov test. ***P « 10−5. For b-e, n = 4 (Epo), n = 3 (G-CSF), n = 5 (control) independent animals.

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