Supplementary Figure 5: Glucose metabolism is diminished in AMPK-silenced GBM cells.
From: AMP kinase promotes glioblastoma bioenergetics and tumour growth
a-h, GSC10 cells were fed with U13C glucose and 13C carbon incorporation in the metabolites of glycolysis and TCA cycle was quantitated by HPLC/Mass spectrometry. Shown are several metabolites of glycolysis (G6P/F6P, G3P), pentose phosphate pathway (6PG, R5P/X5P, S7P) and TCA cycle (Suc, Mal) that show reduced incorporation of 13C glucose carbon at indicated times. Note: Aspartate is generated from the TCA cycle metabolite oxaloacetate. G6P/F6P, glucose 6-phosphate/fructose 6-phosphate; G3P, glucose 3-phosphate; 6-PG, 6-phosphogluconate; R5P/X5P, ribulose 5-phosphate/xylulose 5-phosphate; S7P, sedoheptulose 7-phosphate; Suc; succinate; Mal, malate; Asp, aspartate. Duplicate samples were processed. ** p = 0.003 (M+6 G6P/F6P; M+6 and M+5 6PG); # p = 0.001 (M+ 2 G3P); * p ≤ 0.05 (M+5 R5P/X5P; M+7 and M+6 S7P; M+2 Suc; M+3 Mal; M+3 and M+2 Asp). Source data are available in Supplementary Table 4.
