Supplementary Figure 2: Nucleolar accumulation of ribosomal RNA precursors in senescent cells. | Nature Cell Biology

Supplementary Figure 2: Nucleolar accumulation of ribosomal RNA precursors in senescent cells.

From: Senescence-associated ribosome biogenesis defects contributes to cell cycle arrest through the Rb pathway

Supplementary Figure 2

(a-c) Fluorescence in situ hybridization (FISH) with an antisense or sense probe overlapping the 5.8S(5′) rRNA processing site 2 as shown in Fig. 1c and counterstained with DAPI of (a-b) IMR90 cells at day 20 post-infection expressing (a) H-RASV12 (RAS), (b) PML-IV (PML) or an empty control vector and of (c) uninfected young (passage 25), old (passage 43) cells or confluent arrested for 4 days or arrested by serum starvation for 6 days (Starved), (1 out of 2 independent experiments with similar results). (d-e) Quantification of (d) nucleolar mean area (μm2) relative to vector infected cells and (e) nucleolar fluorescence mean intensity relative to vector-infected cells after FISH for cells marked as in (a-c). Number of nucleoli analysed per condition: Vector (Vect) (n = 174), RAS (n = 100), PML (n = 117), Old (n = 93) and Confluent (n = 166). Error bars indicate SEM. * = p<0.05, using Mann-Whitney U test.

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