Supplementary Figure 8: Cells do not tolerate a significant depletion of RPS14 but RPS14 mRNA levels are downregulated in different types of cancer and increased in nevi. | Nature Cell Biology

Supplementary Figure 8: Cells do not tolerate a significant depletion of RPS14 but RPS14 mRNA levels are downregulated in different types of cancer and increased in nevi.

From: Senescence-associated ribosome biogenesis defects contributes to cell cycle arrest through the Rb pathway

Supplementary Figure 8

(a, c, e and g) Growth curves of (a) IMR90 cells, (c) U2OS cells, (e) PC3 cells or (g) H1299 cells expressing a control shRNA (shNTC) and two shRNA against RPS14 (shRPS14-A and -B). Data are presented as means normalized to day 0 of each condition. (1 out of 2 independent experiments with similar results). (b, d, f and h) Immunoblots for the indicated proteins at day 5 post-infection with a control shRNA (shNTC) and two shRNA against RPS14 (shRPS14-A and -B) in (b) IMR90 cells, (d) U2OS cells, (f) PC3 cells or (h) H1299 cells, (1 out of 2 independent experiments with similar results). (i-m) Studies showing the decrease of RPS14 transcript levels using the Oncomine database selected with the following settings: threshold P-value: 1E-4; fold change: 2; gene rank top 10%. Each box plot shows median RPS14 expression, boxes are upper and lower 25% percentiles, whiskers are upper and lower 10% percentiles, minima and maxima are shown by dots. (For precision see Oncomine database). (i) RPS14 expression in Finak Breast. Comparison of Breast (n = 6) and Invasive Breast Carcinoma (n = 53). (j) RPS14 expression in Haslinger Leukaemia. Comparison of B-Lymphocyte (n = 11) and Chronic Lymphocytic Leukaemia (n = 100). (k) RPS14 expression in Bhattacharjee Lung. Comparison of Lung (n = 17) and Small Cell Lung Carcinoma (n = 6). (l) RPS14 expression in Grasso Prostate. Comparison of Primary Site (n = 59) and Metastasis (n = 35). (m) RPS14 expression in Haqq Melanoma. Comparison of Normal tissue (n = 3), Non-Neoplastic Nevus (n = 9) and Melanoma (n = 25). (n) Summary of studies comparing levels of RPS14, CDK4 and RB1 (RB) transcripts in Skotheim Testis cancers, using the Oncomine database with the following settings: threshold P-value: 1E-4; fold change: 2; gene rank top 10%. Red colour indicated an increase in the tumour whereas blue colour indicated a decrease and n for each tumour type is indicated between parentheses. (For precision see Oncomine database). Unprocessed blots can be found in Supplementary Figure 9.

Back to article page