Supplementary Figure 1: Ribosome biogenesis defects in senescent cells induced by oncogenic ras, short telomeres or the tumour suppressor PML. | Nature Cell Biology

Supplementary Figure 1: Ribosome biogenesis defects in senescent cells induced by oncogenic ras, short telomeres or the tumour suppressor PML.

From: Senescence-associated ribosome biogenesis defects contributes to cell cycle arrest through the Rb pathway

Supplementary Figure 1

(a) Autoradiography for de novo rRNA synthesis after a three hours pulse labelling with [3H]-uridine. Total RNA was extracted after the pulse from IMR90 cells expressing H-RASV12 (RAS) or an empty control vector (Vect) at day 2, 4, 6 and 8 post-infection and compared to total 28S rRNA detected with ethidium bromide (EtBr) under UV light, (1 out of 3 independent experiments with similar results). (b-d) QPCR for senescence biomarkers. Total RNA was extracted from (b-c) IMR90 cells at day 20 post-infection expressing (b) H-RASV12 (RAS), (c) PML-IV (PML) or an empty control vector (Vect) and from (d) uninfected young (passage 24) and old (passage 38) IMR90 cells. RNA was reverse transcribed and analysed by qPCR for the indicated genes. Data were normalized over TBP and HMBS and presented as mean relative to vector-infected cells or young cells, (1 out of 3 independent experiments with similar results).

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