Supplementary Figure 4: RNA-seq analysis from denervated myofibres vs control.

(a) MA-plots showing the fold change and normalized counts for myofibers from 7 days denervated (DEN) muscles compared to control myofibers. Data show results from the mean of 2 animals/group (b) Expression heatmap of genes differentially expressed in myofibers derived from denervated (DEN) muscle compared to myofibers derived from Control (CTR) mice. Gene expression is represented as z-score calculated across the rows. Data show results from the mean of 2 animals/group (c) Upstream Regulators predicted to be differentially activated or inhibited in myofibers from denervated (DEN) myofibers compared to control (CTR) myofibers, by IPA analysis. (d) Top Canonical Pathways altered in myofibers from 7 days Denervated (DEN) muscles, compared to control myofibers. For each plot, x-axis represents the –log of the P-value (right-tailed Fisher’s exact test) and the orange dots on each pathway bar correspond to the ratio of the number of genes in a given pathway that meet the cutoff criteria, divided by the total number of genes belonging to that pathway. (e) Top Metabolic Pathways altered in Myofibers from 7 days Denervated (DEN) muscle compared to control myofibers (see Supp.Fig.3b for further details). For each plot, x-axis represents the –log of the P-value (right-tailed Fisher’s exact test) and the orange dots on each pathway bar correspond to the ratio of the number of genes in a given pathway that meets the cutoff criteria, divided by the total number of genes belonging to that pathway.