Supplementary Figure 2: Increased number of FAPs but not Satellite Cells and Macrophages in denervated muscles. | Nature Cell Biology

Supplementary Figure 2: Increased number of FAPs but not Satellite Cells and Macrophages in denervated muscles.

From: Denervation-activated STAT3–IL-6 signalling in fibro-adipogenic progenitors promotes myofibres atrophy and fibrosis

Supplementary Figure 2

(a) Pax7 (Red) immunofluorescence in control, injured and denervated TA muscle cryosections at the indicated time-point. Laminin (green) and Dapi (white) counterstain is shown in the bottom merged panel. (b) F4/80 (Green) immunofluorescence analysis is shown in control (CTR), injured (CTX) and denervated (DEN) TA muscle cryosections at the indicated time-points. Laminin (Cyan) and Dapi (white) counterstain is shown in the bottom merged panel. (c) Sca-1 (Red) immunofluorescence analysis is shown in control (CTR), injured (CTX) and denervated (DEN) TA muscle cryosections at the indicated time-points. Laminin (Cyan) and Dapi (white) counterstain is shown in the bottom merged panel. In a-c, Scale bar 100 µm. Data shown in a-c represent results from 3 animals/group, 3 independent experiments were performed. Quantification was shown in (d-e-f). Values represent mean ±s.d. *P<0.05, **P<0.01; by one-way ANOVA (n=3 animals/group).

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