Supplementary Figure 8: Unprocessed scans of raw blots. | Nature Cell Biology

Supplementary Figure 8: Unprocessed scans of raw blots.

From: RAS nucleotide cycling underlies the SHP2 phosphatase dependence of mutant BRAF-, NF1- and RAS-driven cancers

Supplementary Figure 8

(a) SHP2 IP from Fig. 5c, (b) GAB1 IP from Fig. 5d, and (c) membrane fractionation from Fig. 5e. (d) Effect of RMC-4550 on RAS-GTP levels from Supplementary Figure 1b, (e) Effect of SHP2 variants on pERK in H508 cells from Supplementary Figure 2b, (f) Effect of SHP2 variants on pERK in H1838 cells from Supplementary Figure 2d, (g) Effect of SHP2 variants on pERK in H358 cells from Supplementary Figure 2f. (h) Effect of NF1 shRNA knockdown on RAS-GTP levels from Supplementary Figure 3a, and (i) RAS-GTP levels from Supplementary Figure 3c, (j) Effect of SHP2 and SOS1 shRNA knockdown in RAS-GTP levels from Supplementary Figure 3d, (k) Effect of RMC-4550 on RAS-GTP and pERK levels in RAS-less MEFs from Supplementary Figure 4b, (l) Effect of SOS or SOS-F expression on pERK from Supplementary Figure 4e, (m) NF1 levels in TH77 PDX model from Supplementary Figure 7a. Note that panels (d) through (e) are located in additional pages.

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