Supplementary Figure 3: Characterization of extra-embryonic ectoderm cells.
From: Sequential formation and resolution of multiple rosettes drive embryo remodelling after implantation
(a) Images of different Z slices (0.8 um) from a representative E5.5 (Stage III) embryo. Extra-embryonic ectoderm (ExE) rosettes are outlined with a dashed line. Arrows denote rosettes’ centre. Image representative of 20 embryos (b) Representative example of an ExE rosette (dashed outline) found in E5.5 embryo as revealed by cell morphology (E-cadherin) and polarization (ZO-1). Orthogonal views (XZ, YZ) of the embryo are displayed on the right and the bottom of the image with the segmented rosette as viewed in the respective orthoslice. Image representative of 20 embryos. (c) Cell aspect ratio comparison of outside, inside vs inside cells that contribute to rosettes (Inside cells, R). Two sided unpaired student t-test;****p<0.0001;mean± SEM; n=50 inside cells and n=47 inside rosette cells. (d) Plot of long and short axis. Two separate clusters are indicated (magenta and grey outlines). Cluster shown with magenta consists of outside cells and inside cells contributing to rosettes (inside cells R). Cluster shown with grey consists of inside cells that do not contribute to rosettes. Blue dashed line: y=x. n=50 outside and inside cells and n=47 inside rosette cells. Scale bars=20um.
