Supplementary Figure 8: Cell intercalation after initial cavities fusion results in the formation of a pseudostratified epithelium.
From: Sequential formation and resolution of multiple rosettes drive embryo remodelling after implantation
(a)ExE acquires a pseudostratified epithelium morphology shortly after unification of the cavities. Image representative of 10 embryos. (b)After cavities fusion ExE cells show intercalative behaviour (green and yellow asterisks). Image representative of 5 embryos. (c) 3 different optical Z slices (0.6um) of a representative example of an E5.75(stage IV) embryo. Dashed outline in left panel shows pro-amniotic cavity spanning through EPI and ExE. In different Z slices (middle and right panel) ExE cells intercalating towards the basement membrane can be identified. Polarized pMLC localization displays the polarized nature of this event. Insets in middle and right panel show magnified fluorescent intensity color coded images of ExE intercalating cells. Image representative of 5 embryos. Scale bars=20um.
