Supplementary Figure 1: Insulin production by α-cells after β-cell ablation in islets transplanted under the kidney capsule. | Nature Cell Biology

Supplementary Figure 1: Insulin production by α-cells after β-cell ablation in islets transplanted under the kidney capsule.

From: Pancreatic islet-autonomous insulin and smoothened-mediated signalling modulate identity changes of glucagon+ α-cells

Supplementary Figure 1

(a) Immunofluorescence staining of insulin (green) and glucagon (red) in RIP-DTR islets transplanted under the kidney capsule, either before (‘no DT’) or after (‘DT’) β-cell ablation. The scatter graph reports a quantification of the β-cell ablation efficiency, measured as the number of insulin-containing cells remaining per islet section in non-treated and DT-treated animals, 10 days later. (mean +/− s.e.m., n = 40 islet sections per condition. (b) Experimental design of Exp. #4, where islets were transplanted in the kidney capsule of immunocompromised mice (SCID) either mixed together or at 2 separated sites. (c) Proportion of YFP-traced α-cells expressing insulin 1-month post-DT in mixed RIP-DTR+WT islets compared to RIP-DTR islets from 2-sides graft. Data are shown as mean +/− s.d.; n = 3 biologically independent animals per condition. (d) Immunofluorescence staining of mCherry (magenta), YFP (green), insulin (red) in transplanted mixed RIP-DTR+WT islets 1-month post-DT. Scale bars: 20 µm. Experiment repeated independently 3 times. See Supplementary Table 1 as source data.

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