Supplementary Figure 2: β5-positive reticular adhesions lack consensus adhesome components. | Nature Cell Biology

Supplementary Figure 2: β5-positive reticular adhesions lack consensus adhesome components.

From: Reticular adhesions are a distinct class of cell-matrix adhesions that mediate attachment during mitosis

Supplementary Figure 2

(a-m) Confocal images of U2OS cells plated for 3 h on vitronectin and immuno-labeled against integrin β5 and: the alpha V (αV) subunit of the αVβ5 heterodimer (a); consensus adhesome components [paxilin (b), FAK (c), zyxin (d), kindlin 2 (e)]; integrin β5-binding partners [CSK (genetically encoded GFP-CSK) (f), ICAP1 (g), DOK1 (h)]; phospho-tyrosine (i); and cytoskeletal proteins [F-actin (j), cytokeratin (k), beta (β)-tubulin (l), vimentin (m)]. (n) Confocal image of U2OS cells plated for 3 h on laminin (ECM ligand not bound by αVβ5) and immuno-labeled for integrin β5 and vinculin. (o) Confocal images of an unpermeabilised U2OS cell expressing β5-2GFP, immuno-labeled for β5. All images representative of results from at least n = 3 biologically independent experiments. Scale bars: 10 µm. Boxed areas shown at higher magnification in lower right corners

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