Supplementary Figure 4: Validation of reticular adhesion proteins identified by mass spectrometry. | Nature Cell Biology

Supplementary Figure 4: Validation of reticular adhesion proteins identified by mass spectrometry.

From: Reticular adhesions are a distinct class of cell-matrix adhesions that mediate attachment during mitosis

Supplementary Figure 4

(a,b) Gene-ontology analysis of U2OS steady state adhesion proteins (DMSO treated; focal and reticular adhesions) showing terms from Biological Process (a) and KEGG pathway analysis (b) significantly enriched over whole cell proteome (based on data from n = 3 biologically independent mass spec experiments). P-values were derived from EASE scores calculated using a modified Fishers Exact test with Holm-Bonferroni correction from multiple tests using the DAVID annotation system. (c-g) Confocal images of U2OS β5 V cells attached to vitronectin (VN)-coated surfaces and immuno-labeled against EPS15L1 (c), HIP1R (d), or WASL (e), Intersectin-1 (f), and ARP3 (g). All images representative of results from at least n = 3 biologically independent experiments. Scale bars: C-E = 10 µm; F-G = 5 µm

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