Supplementary Figure 7: Mitotic reticular adhesions lack canonical adhesome components. | Nature Cell Biology

Supplementary Figure 7: Mitotic reticular adhesions lack canonical adhesome components.

From: Reticular adhesions are a distinct class of cell-matrix adhesions that mediate attachment during mitosis

Supplementary Figure 7

(a-e) Confocal images of mitotic U2OS cells (yellow dashed outlines) plated on VN and labeled for integrin β5 and: integrin β3 subunit (a); and consensus adhesome components [talin (b), vinculin (c), paxillin (d), zyxin (e)]. (f-g) Confocal images of mitotic U2OS cells plated on 10μg/ml laminin (f) or fibronectin (g) immuno-labeled against integrin β5 and integrin β1 subunits. Images in A-K representative of at least n = 3 biologically independent experiments. (h-k) Alternate views of actin-positive retraction filaments (phalloidin-labeled) formed during mitosis and terminating at integrin β5-2GFP-positive adhesion complexes (K cropped from yellow box in J; see Supplementary Movie 9). Images in H-K representative of n = 2 biologically independent experiments. Scale bars:10 µm except in K; 5 µm

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