Supplementary Figure 8: Disruption of HeLa cell mitosis by integrin β5 siRNA.

(a) Hela cells transfected with control siRNA showing normal cell division (see Supplementary Movie 10). (b) Three example microscopy time series representative of different effects of depleting β5 on cell division: i) cell remains round for an extended time before eventually dividing, often with incomplete cytokinesis (see Supplementary Movie 11). ii) cell repeatedly rounds up and respreads without dividing (see Supplementary Movie 12). iii) cell appears to divide but before cytokinesis merges back into a single bi-nucleate cell (see Supplementary Movie 13. (c) Hela cell treated with β5 siRNA and rescued with β5-EGFP (see Supplementary Movie 14). (a-c) Images were taken at 10 min intervals. Arrows point to mitotic cells of interest. Images representative of n = 3 biologically independent experiments. (d) Representative immunoblot of endogenous and β5-EGFP expression in control and knockdown cells. Unprocessed blot in Supplementary Fig 9. Scale bars = 50 µm