Supplementary Figure 1: β5-positive, talin- or F-actin-negative structures found in multiple cell lines. | Nature Cell Biology

Supplementary Figure 1: β5-positive, talin- or F-actin-negative structures found in multiple cell lines.

From: Reticular adhesions are a distinct class of cell-matrix adhesions that mediate attachment during mitosis

Supplementary Figure 1

(a) A549 lung cancer cells and A375 melanoma cells plated on glass coverslips for 72 h. Confocal images of immuno-fluorescently labeled integrins αVβ3 (LM609), β1 (9EG7) and αVβ5 (15F11). (b) U2OS cells plated on glass coverslips for 24 and 48 h. Confocal images of immuno-fluorescently labeled integrin β5 and vinculin. (c,d,e) The indicated human-, mouse- and hamster-derived cancer cell lines (c), immortalized diploid cell lines (d) and primary cells (e) were found to contain central β5-positive, talin- and F-actin-negative structures equivalent to those first observed in U2OS cells. Confocal images of cells replated for 3 h on 10 µg/ml vitronectin and immuno-labeled for integrin β5 and either talin or F-actin, as indicated. All images representative of results from at least n = 3 biologically independent experiments. Scale bars: 10 µm

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