Supplementary Figure 4: CRISPR tiling screening performed across mSWI/SNF subunit genes in SYO-1 cells reveals subunit- and subunit domain- specific dependencies.

(a). Box plot of dropout scores for guides targeting the SS18 gene or non-targeting control in CRISPR tiling screens in SYO-1. Box represents interquartile range (IQR), bar in center shows data median. Minima and maxima shown extend to from the box +/− 1.5*IQR with data falling outside of that range shown as points. (SS18: n = 166, Control: n = 200). (b). CRISPR tiling screening performed across the SS18 gene in SYO-1 cells. (c,d). CRISPR tiling screening performed across the SMARCB1 (c) and SMARCE1 (d) genes in SYO-1 cells. (e). Box plot of dropout scores for guides to SMARCD family paralogs or non-targeting control in CRISPR tiling screen in SYO-1. Box represents interquartile range (IQR), bar in center shows data median. Minima and maxima shown extend to from the box +/− 1.5*IQR with data falling outside of that range shown as points. (SMARCD1: n = 147, SMARCD2: n = 167, SMARCD3: n = 137, Control: n = 200). (f). CRISPR tiling screening performed across SMARCD1 gene indicating no specific domain dropout. (g). Box plot of dropout score for guides to SMARCC family paralogs or non-targeting control in CRISPR tiling screen in SYO-1. Box represents interquartile range (IQR), bar in center shows data median. Minima and maxima shown extend to from the box +/− 1.5*IQR with data falling outside of that range shown as points. (SMARCC1: n = 314, SMARCC2: n = 430, Control: n = 200). (h). CRISPR tiling screening performed across the SMARCC1 gene indicating no specific domain dropout. (i). Immunoprecipitation of mammalian GLTSCR1 full-length (GLTSCR1-FL) and GLTSCR1 N-terminal deletion (G1-Ndel) followed by immunoblot (n = 2 biologically independent experiments).