Supplementary Figure 1: Inhibiting proliferation in most stem cell by blocking cell cycle does not affect hair follicle growth rate.

(a), Immunofluorescence staining of early Anagen hair follicles for cell cycle markers Ki67 and BrdU showing the H2BGFP+ cells that also express Cdkn1b in the proliferation inhibition experiments (Lgr5-CreER; R26-flox-STOP-tTA; tetO-Cdkn1b; pTRE-H2BGFP; K14-H2BmCherry) have blocked cell cycle. Images representative of 3 mice. Scale bar, 50 µm. (b), No significant growth rate difference between the Cdkn1b-overexpressing hair follicles carrying non-inhibited stem cells only at a certain position. Plot shows the mean±SD from 3 mice (n=10, n=9 and n=15 hair follicles in each group). ns, not significant, p=0.2385, 0.5210 and 0.4856 when comparing each two groups. Two-sided unpaired t-test was used to calculate p value. Statistical source data are provided in Supplementary Table 1.