Supplementary Figure 1: Loss of G9a does not alter overall levels of H3K4me1, H3K4me3, H3K27me3 or H3K27Ac. | Nature Cell Biology

Supplementary Figure 1: Loss of G9a does not alter overall levels of H3K4me1, H3K4me3, H3K27me3 or H3K27Ac.

From: Loss of G9a preserves mutation patterns but increases chromatin accessibility, genomic instability and aggressiveness in skin tumours

Supplementary Figure 1

(a) Schematic of generation of G9acKO mice. (b) Immunofluorescence for the indicated histone marks (green) on WT and G9acKO skin. Nuclei are counterstained with DAPI (blue). White dashed line marks the epidermis. Yellow dashed line indicates the dermal papilla. Scale bar, 100 µm. Images shown are representative of n = 4 (WT) and n = 4 (G9acKO) independent animals. (c) Representative immunoblots for the indicated histone marks on WT or G9acKO epidermal cells. (d) Quantification of (c). Data shown are mean ± s.e.m. of individual animals. WT, n = 5 independent animals for H3K4me1 and H3K27me3, n = 4 independent animals for H3K4me3, n = 3 for H3K27Ac. G9acKO, n = 6 independent animals for H3K4me1 and H3K27me3, n = 5 independent animals for H3K4me3, n = 4 for H3K27Ac. Two-sided Student´s t-test. (e) Sorting strategy for isolation of HFSCs and EpSCs. (f) Location of differentially open ATAC-seq regions of WT or G9acKO EpSCs or HFSCs annotated by genomic feature. Data shown are mean ± s.e.m. of individual animals. WT, n = 3 independent animals; G9acKO, n = 3 independent animals. Two-sided Fisher´s exact test with FDR < 0.05 to correct for multiple testing. (g) Location of differentially open ATAC-seq regions of WT or G9acKO EpSCs or HFSCs with respect to the closest TSS. Data shown are mean ± s.e.m. of individual animals. WT, n = 3 independent animals; G9acKO, n = 3 independent animals. Two-sided Fisher´s exact test with FDR < 0.05 to correct for multiple testing. EpSCs, epidermal stem cells; HFSCs, hair follicle stem cells; TSS, transcription start site. See Supplementary Table 8 for source data

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