Supplementary Figure 2: G9a is dispensable for epidermal development and maintenance. | Nature Cell Biology

Supplementary Figure 2: G9a is dispensable for epidermal development and maintenance.

From: Loss of G9a preserves mutation patterns but increases chromatin accessibility, genomic instability and aggressiveness in skin tumours

Supplementary Figure 2

(a) Heatmap showing unsupervised hierarchical clustering of top 1000 expressed genes between WT and G9acKO HFSCs or EpSCs isolated from homeostatic epidermis. (b) Venn diagrams indicating genes commonly down- or up-regulated in G9acKO HFSCs or EpSCs as compared to WT. (c,d) Representative FACS plots showing frequency of CD45-positive cells within WT or G9acKO epidermis, with quantification. Data shown are mean ± s.e.m. WT, n = 5 independent animals; G9acKO, n = 4 independent animals. Two-sided Student´s t-test. (e,f) Immunostaining for CD3 of WT and G9acKO skin to assess infiltration of lymphocytes, with quantification. Scale bar, 50 µm. Data shown are mean ± s.e.m. WT, n = 5 independent animals; G9acKO, n = 5 independent animals. Two-sided Student´s t-test. (g) Haematoxylin and eosin staining on WT or G9acKO skin at 15 or 90 weeks of age. Scale bar, 50 µm. (h) Quantification of interfollicular epidermis thickness of WT and G9acKO skin at 15 or 90 weeks of age. Data shown are mean ± s.e.m. WT, n = 3 independent animals; G9acKO, n = 3 independent animals. Data points indicate fields of view. Two-sided Student´s t-test. (i) Distance between hair follicles of WT and G9acKO skin at 15 or 90 weeks of age. Data shown are mean ± s.e.m. WT, n = 3 independent animals; G9acKO, n = 3 independent animals. Data points indicate fields of view. Two-sided Student´s t-test. (j,k) Immunostaining for Ki67 of WT and G9acKO skin to assess proliferation rate, with quantification of positive cells within hair follicles or interfollicular epidermis. Scale bar, 50 µm. Data shown are mean ± s.e.m. WT, n = 3 independent animals; G9acKO, n = 3 independent animals. Data points indicate fields of view. Two-sided Student´s t-test. (l,m) Representative FACS plots showing frequency of HFSCs and EpSCs populations within WT or G9acKO epidermis with quantification. Data shown are mean ± s.e.m. WT, n = 13 independent animals; G9acKO, n = 13 independent animals. Two-sided Student´s t-test. EpSCs, epidermal stem cells; HFSCs, hair follicle stem cells. See Supplementary Table 8 for source data

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