Fig. 5
From: Author Correction: ZSCAN10 expression corrects the genomic instability of iPSCs from aged donors
Evaluation of ZSCAN10 function in DNA damage response and genomic integrity in human A-hiPSCs. a, Immunoblots showing the levels of pATM and β-actin proteins with three imported A-hiPSC clones with known abnormal cytogenetic signature. b, qPCR of ZSCAN10. The error bars indicate s.e.m. of two replicates with three independent clones (n = 6) in each sample group, except three biological replicates (n = 3) in the sample of A-iPSC-JA and A-iPSC-LS. Statistical significance was determined by two-sided t-test followed by post hoc Holm–Bonferroni correction for a significance level of 0.05; *indicates significant. Note that these data are also presented in Supplementary Fig. 6i. c, qPCR of GSS. Statistical significance was determined by two-sided t-test followed by post hoc Holm–Bonferroni correction for a significance level of 0.05; *indicates significant. The error bars indicate s.e.m. of two replicates with three independent clones (n = 6) in each sample group, except three biological replicates (n = 3) in the sample of A-iPSC-JA and A-iPSC-LS. (Note that these data are also presented in Supplementary Fig. 6j.) d, Immunoblots showing the levels of pATM and β-actin in five independent clones of A-hiPSCs, five independent clones of Y-hiPSCs and five clones of A-hiPSCs expressing ZSCAN10. e, Immunoblot showing impaired ATM DNA damage response in Y-hiPSCs with ZSCAN10 shRNA expression in three independent clones after phleomycin treatment (2 h, 30 μg ml−1). f, Copy number profiling analysis of Y-hiPSCs with ZSCAN10 shRNA expression in four independent clones43. Unprocessed original scans of blots are shown in Supplementary Fig. 7.
