Supplementary Fig. 4: LRP6-KO HEK293T STF cell line validation.
From: EGFR is required for Wnt9a–Fzd9b signalling specificity in haematopoietic stem cells
a, WISH for cmyb at 40 hpf in zebrafish injected with suboptimal MO dosages (0.1 ng wnt9a and 0.5 ng fzd9b). For quantification, see Fig. 2g. Scale bar, 30 µm. b, Quantification of the HEK293T cell STF assay with zebrafish Wnt9a, Fzd9b and Lrp6; n = 3 biological replicates each. Statistical analyses are compared to Wnt9a alone. c, Quantification of the HEK293T cell STF assay screen with mouse Wnt3a in WT and LRP6-KO cells; n = 3 biological replicates each. Statistical analyses were done by ANOVA as indicated. d, Quantification of the HEK293T cell STF assay with 500 nM CHIR in WT and LRP6-KO cells; n = 3 biological replicates each. In all graphs, the dots represent biological replicates from one experiment, the bars represent the mean and the error bars represent the standard deviation. Statistical analyses were done by ANOVA compared to controls as indicated. All STF assays were repeated independently with a similar trend.
