Supplementary Fig. 1: Fzd screening.
From: EGFR is required for Wnt9a–Fzd9b signalling specificity in haematopoietic stem cells
a, Schematic of strategy to screen for fzd expression in endothelial cells as marked by fli1a-GFP at 16.5 hpf. b, Expression of all 14 fzd transcripts in Fli1a+ cells at 16.5 hpf; n = 1 experiment shown as representative data from qPCR on pooled sorted cells; trend reproduced in 3 independent experiments. Bars represent the mean of three technical replicates. c, In the Wnt-off state, the STF reporter is silent and luciferase is not expressed. d, In the presence of a Wnt signal, β-catenin is translocated to the nucleus, where it activates the STF reporter and luciferase is expressed. e, Quantification of the HEK293T cell STF assay with Wnt9a and Fzd8a or Fzd9b in the context of STF or FOP:FLASH reporters; n = 3 biological replicates each. f, Quantification of the HEK293T (FZD1, FZD2 and FZD7 KO) cell STF assay with zebrafish Wnt3a and zebrafish Fzd8a, Fzd9a, Fzd9b or human FZD7; n = 3 biological replicates each. In all STF assays, the bars represent the mean, the error bars represent the standard deviation and the dots represent individual biological replicates; one-way ANOVA with Dunnett’s test. All STF assays were repeated independently with a similar trend.
