Supplementary Figure 4: Non-centromeric CENP-A binding peaks overlap with active transcription marks.

(a,b) The chromatin features of CENP-A^TAP (a) and CENP-A^LAP (b) non-centromeric preferential sites were analysed by intersecting SICER peaks ≥ 5-fold supported between two replicates with publicly available ENCODE datasets for histone modification profiles in HeLa S3, that represent modifications typically associated with transcription activation or repression. The experiment was performed one time, except for DNase I and H3K27me3 for which there are 2 ENCODE datasets available, and therefore for DNase I and H3K27me3 the experiment was repeated twice independently with similar results. Statistics source data for Supplementary Fig. 4a, b can be found in Supplementary Table 4. (The sum of ectopic CENP-A^TAP sites at active or repression marks is more than 100%, the result of overlap between H3K9me3 and active transcription marks.) (c,d) The chromatin features of sites of preferential, non-centromeric CENP-A binding were analysed for histone modification profiles associated with transcription activation or repression in HeLa S3 cells by intersecting SICER peaks ≥ 5-fold found in previously published CENP-A ChIP-seq datasets in HT1080⁷ (c) and HuRef⁸ (d) cell lines with publicly available ENCODE datasets for histone modification profiles in HeLa S3. For HT1080b (c) the experiment was performed one time, except for DNase I and H3K27me3 for which there are 2 ENCODE datasets available, and therefore for DNase I and H3K27me3 the experiment was repeated twice independently with similar results. For HuRef (d), The experiment was performed four times, except for DNase I and H3K27me3 for which there are 2 ENCODE datasets available, and therefore for DNase I and H3K27me3 the experiment was repeated independently eight times with similar results. Source data for a-d can be found in Supplementary Table 4.