Supplementary Figure 5: Loss of GCN2 does not affect MYC driven lymphomagenesis but loss of GCN2 combined with inhibition of PERK promotes survival of MYC driven lymphoma bearing mice.

a. Representative images of genotyping PCR products of mice. PCR was performed more than three times independently. b. Kaplan-Meier analysis for overall survival of Eµ-Myc/+; Gcn2^+/+ (n=28), Eµ-Myc/+; Gcn2^+/- (n=27) and Eµ-Myc/+; Gcn2^-/- mice(n=30). Kaplan-Meier curves were analyzed by two-tailed log-rank test. c. Representative western blot assessing ISR signaling in B cells isolated from tumor bearing mice or WT litter mates. Immunoblot is a representative of 3 independent experiments showing similar results. d. Schematic showing the treatment regimen performed in allograft model of lymphomagenesis. 2 million lymphoma cells were injected via tail vein into mice and LY-4 treatment was started three days after tumor injection. e. Body weight of mice injected with lymphoma cells during LY-4 treatment, (n=8 per each group). f. Pancreas weight of the mice in panel e. n=7 mice per group. Error bars represent mean ± SD, two tailed student t-test. g. Kaplan-Meier analysis for overall survival of mice treated with either vehicle or LY-4 (n=8 per each group). Kaplan-Meier curves were analyzed by two-tailed log-rank test. h. Representative immunoblot of ISR signaling examined in tumors isolated from the indicated groups in panel d at the end of the experiment. i. Quantification of immunoblots for p-eIF2α, including panel c. n = 3 for GCN2 +/+: Veh, n = 6 for GCN2 +/+: LY-4, n = 7 for GCN2 -/-: Veh and GCN2 -/-: LY-4. Error bars represent mean ± SD, two tailed student t-test. Unprocessed scans of blots are shown in Supplementary Fig 8. Unprocessed scans of blots are shown in Supplementary Fig 8.