Supplementary Figure 6: Acute deletion of ATF4 significantly delays MYC driven lymphomagenesis.
From: ATF4 couples MYC-dependent translational activity to bioenergetic demands during tumour progression
a. Schematic showing insertion of LoxP sites in mouse Atf4 locus. Exon 2 and 3, which code for ATF4 protein are deleted after Cre recombinase mediated excision. b. Genotyping PCR products of mice used in in vivo experiments. 1=Eμ-Myc/+, Rosa26CreERT2/+, ATF4+/+ 2= Eμ-Myc/+, Rosa26CreERT2/+, ATF4fl/fl, 3= WT. c. Kaplan-Meier analysis for lymphoma-free survival of mice bearing Eµ-Myc; ATF4+/+ lymphoma treated with either vehicle (veh) or tamoxifen (tam) for 5 days, n=10. Kaplan-Meier curves were analyzed by two-tailed log-rank test. d. mRNA expression of ATF4 from Fig 4d, showing a significant reduction of ATF4 mRNA in B cells treated with tamoxifen, n=3, mice per group. Error bars represent mean ± SD, two tailed student t-test. e. mRNA expression of ATF4 at the endpoint mice treated with vehicle or tamoxifen whose survival is shown in Fig 4b, c. n=3, mice per group. Error bars represent mean ± SD, two tailed student t-test.
