Supplementary Figure 2: ATF4/MYC ChIP-seq.
From: ATF4 couples MYC-dependent translational activity to bioenergetic demands during tumour progression
a. Previously reported ATF4 targets occupied by ATF4 at 8hr of MYC induction within 5kb from TSS. b. Functions and pathways significantly enriched among genes bound by ATF4 within 5kb from TSS and upregulated at 8 hours of MYC activation, E=enrichment, FDR=false discovery rate, UP=Uniprot, MF=molecular function, BP=biological process. Two independent biological replicates were used for ChIP-seq showing similar result. P-values were calculated by Ingenuity Pathway Analysis using right-tailed Fisher Exact Test with FDR values indicating correction for multiple testing. c. ChIP-qPCR validation of ATF4 target genes (left) and genes bound by both ATF4 and MYC (right). Technical replicates, n=3.
