Supplementary Figure 4: Cholesterol is colocalized with Tspan4 on migrasome-like structures in in vitro systems.
From: Migrasome formation is mediated by assembly of micron-scale tetraspanin macrodomains
Z-stack images of an in vitro-reconstituted Tspan4-containing GUV labeled with Topfluor-cholesterol and stained by Cy5-SE to label Tspan4. Images were acquired by confocal microscopy, then mean intensity Z-projections were generated by ImageJ. Purple, proteins; green, Topfluor-cholesterol; red, Rhodamine-PE. Images are representative of 34 tethers from 4 independent experiments. Scale bar, 2.5 µm.
