Supplementary Figure 6: Unprocessed Blots and Gel.
From: Migrasome formation is mediated by assembly of micron-scale tetraspanin macrodomains
a, Unprocessed blots related to Fig. 1d. Verification of Tspan4-GFP protein expression levels in different cell lines by western blot. The sections presented in Fig. 1d were labeled by red squares. b, Unprocessed gel related to Fig. 4a. Recombinant Tspan4-GFP was analyzed on an SDS-PAGE gel and stained by Coomassie Brilliant Blue. The section presented in Fig. 4a was labeled by red square. c, Unprocessed blots related to Supplementary Fig. 1b. Cells with stable Tspan4-GFP expression were treated with or without deglycosylation mix, then lysed and analyzed by western blot. The sections presented in Supplementary Fig. 1b were labeled by red squares.
