Supplementary Figure 6: Examination of the effect of 5FU treatment on Notch signaling.

a, Notch1 activity as assessed by NICD immunostaining. Scale bars, 100 μm. b, RNAscope in situ hybridization shows changes in expression patterns of Ank2 (Class 2) and Ccnb1 (Class1) during recovery from 5FU. Scale bars, 100 μm. c, RNAscope in situ hybridization shows changes in expression patterns of Notch1 and target genes during recovery from 5FU. Scale bars, 100 μm. d, Heatmap showing the average expression of Notch signaling pathway components per Class 3 populations in control and recovery day 3 states.