Supplementary Figure 4: Custom Nextera sequencing strategy and data quality of sc-itChIP libraries.
From: Profiling chromatin states using single-cell itChIP-seq
a, T5 and T7 barcodes are introduced during Tn5 complex assembly with transposon oligonucleotides, and i5 and i7 barcodes are introduced during PCR enrichment for sequencing library preparation on Illumina Hiseq 2500 before size selection with AMPure XP beads. Modified from the study by Amini et al. 19. b, Size distribution of single-cell itChIP-seq data from two independent experiments. c, Scatter plots showing genome-wide correlation of two independent experiments as in (b). ChIP signals were measured in non-overlapping 5 kb windows genome wide. Pearson correlation coefficient (r) is shown in the box. d, Distribution of unique, non-duplicated reads per cell in two independent experiments of 1:10 mixed ESCs and differentiating ESCs (EpiLCs) after filtering single cells of potential barcodes with <3,000 reads.
