Supplementary Figure 5: Clustering of sc-itChIP data of Nkx2-5 lineages from E7.75 and E8.25 hearts.
From: Profiling chromatin states using single-cell itChIP-seq
a, Figure exemplifying the gating strategy. FACS scheme for the isolation of E7.75 and E8.25 Tomato positive NKX2.5 lineage cells using fluorescent-minus-cell as controls. The gate shown on each plot is indicated above the plot. b, c, MDS plot of single cells colored by clusters (b) and embryonic stages (c). CP, cardiac progenitor cells; EC, endothelial cells; CM, cardiomyocytes. Cells were clustered using the method in Preissl at al23. d, Scatter plot showing the enhancer activity of feature genes. The representative marker genes were selected for the cell type validation of CP, EC and CM respectively. Red and grey indicate on/off of enhancer activity in each single cell. Single cells were from two biologically independent experiments.
