Supplementary Figure 4: Ectopically expressing XLF or PAXX could not suppress the mobility of the broken ends in the IFFO1-/- cells. | Nature Cell Biology

Supplementary Figure 4: Ectopically expressing XLF or PAXX could not suppress the mobility of the broken ends in the IFFO1-/- cells.

From: The nucleoskeleton protein IFFO1 immobilizes broken DNA and suppresses chromosome translocation during tumorigenesis

Supplementary Figure 4

a, Immunoblotting show the expression level of IFFO1 in the IFFO1-/- cells transfected with vary versions of IFFO1. b, c, Immunoblottings show the knockdown efficiency of XRCC4 (b) and Lamin A/C (c). Two shRNAs were used for XRCC4 (shX4_1/2) and Lamin A/C (shLM_1/2), respectively. d, Immunoblotting show the expression level of XLF and PAXX in the IFFO1-/- cells. e, MSD of the mCherry–53BP1TD foci in the IFFO1-/- cells transfected with XLF or PAXX. Means with SEMs of every time point are showed. The numbers of traces pooled from two independent experiments are indicated. All immunoblots are representative of three independent experiments; unprocessed scans of immunoblots are shown in Supplementary Fig. 9. Statistical source data including precise P values are shown in Supplementary Table 5.

Back to article page