Supplementary Figure 6: IFFO1 expression is downregulated by its promoter methylation in multiple tumour types.
a, The correlation between the IFFO1 expression and its methylation at promoter. Correlation Coefficient (R) and P Value (PR) were calculated using Pearson Correlation; n = 16 cancer types. b, Relative expression in tumour vs. normal tissue in multiple tumour types. Gene expression data in TCGA were accessed through The UCSC Cancer Genomics Browser (https://genome-cancer.ucsc.edu/). c, Heatmaps of DNA methylation at IFFO1 promoter in different cell lines. Methylation of 18 CpG sites in chr.12: 6,556,011-6,556,233 (GRCh38.p12) were determined. Red and cyan squares denote methylated and non-methylated CpG sites, respectively. White squares denote CpG sites, which were not covered by sequencing. d, mRNA of IFFO1 was measured by qPCR. GAPDH mRNA was measured as a control. Means with SD from n = 3 independent experiments were present. Two-tailed t-tests were used for the analysis. e, Correlation between IFFO1 expression and its promoter methylation in these cell lines examined. Analysis was performed using Pearson Correlation; n= 9 cell lines. f, IFFO1 mRNA level after azacytidine treatment. The EKVX cells were treated with azacytidine for 3 days before harvest. Means with SD from n = 3 independent experiments were present. Two-tailed t-tests were used for the analysis. Statistical source data including precise P values are shown in Supplementary Table 5.
