Supplementary Figure 5: PtdIns4P and ORP1L localize to mutually exclusive phagosomal microdomains. | Nature Cell Biology

Supplementary Figure 5: PtdIns4P and ORP1L localize to mutually exclusive phagosomal microdomains.

From: Phagolysosome resolution requires contacts with the endoplasmic reticulum and phosphatidylinositol-4-phosphate signalling

Supplementary Figure 5

a) Representative time-lapse gallery of confocal micrographs showing mutual exclusion between PtdIns4P (detected with mCh-2xP4M) and GFP-ORP1L. b) Confocal slice of RAW macrophages expressing either soluble GFP (left) or GFP-ORP1L (right) after 4.5 h of phagocytosis of TAMRA-labeled RBC; insets show magnifications of the green channel of the dotted white boxes. Scale bars = 10 µm.

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