Supplementary Figure 4: Conservation analysis of the lamin A/C acetyl(Lys)sites.
From: The NSL complex maintains nuclear architecture stability via lamin A/C acetylation
a) Left; Representative immunostaining of lamin B1 (green) and OCT4 (red) in control and Mof KO (4 days) ESCs. dsDNA was counterstained using DAPI. Scale bars, 5μm. Right; Quantification of nuclear abnormalities in control and Mof KO ESCs. n=2 biological replicates. b) Phylogenetic tree of lamin A/C in the indicated organisms based on complete protein sequences. The maximum-likelihood tree was reconstructed using PhyML. (Uniprot annotations; P02545 – Homo sapiens, P48678 - Mus musculus, P13648 - Gallus gallus, B3DKC5 - Danio rerio, Q60HF0 - Macaca fascicularis, Q21443 - Caenorhabditis elegans, F1MYG5 - Bos Taurus, Q03427 - Drosophila melanogaster). c) Vertebrate lamin A/C protein sequence alignment. The lysine (K) residues are highlighted in red. The coiled-coil domain is framed and the detected acetyl(K)sites from the MEFs SILAC are marked with asterisks. Alignment was performed using the SeaView software employing the clustal omega algorithm.
