Supplementary Figure 6: Sensitivity of lamin A/C acetylation to HDAC inhibition and representative genomic DNA read-depth plots for control, Mof KO and K311R lamin A MEFs.

a) Immunoblotting on whole cell extracts of control and Mof KO upon panobinostat (200nM) and bufexamac (5μM) treatment. b) Quantification of nuclear abnormalities in control and Mof KO MEFs treated with the indicated panobinostat and bufexamac concentrations; Ordinary one-way ANOVA on n=3 independent biological replicates. c–e) Normalized read-depth along the genome for typical WT control primary MEFs. Three different cells are shown. f) Normalized read-depth along the genome for a Mof KO MEF exhibiting chromothripsis, shown in Fig. 4i. g) Normalized read-depth along the genome for a K311R lamin A MEF exhibiting chromothripsis, shown in Fig. 4j. h) Normalized read-depth plots and CBS segmentation results for selected Mof KO cells. CBS segments were further computationally processed (Methods) to generate high confidence segments used in Fig. 4e, f. i) Immunoblotting on subcellular fractions of MEFs expressing the indicated OST-lamin A derivatives. j) Normalized read-depth plots and CBS segmentation results for selected K311R lamin A MEFs. CBS segments were further computationally processed (Methods), to generate high confidence segments used in Fig. 4g, h. Uncropped blots of a, i can be found in Supplementary Fig. 9.