Supplementary Figure 7: In depth characterization of the nuclear abnormalities epigenetic landscape.

a) Quantification of H3K27me3 and H3K27ac normalized to DAPI in the main nuclei, nuclear blebs and micronuclei of Mof KO (4 days); Nonparametric one-way ANOVA followed by a Kruskal-Wallis test. b) Representative immunostaining of H4K8ac (green) in Mof and Kansl2 KO (4 days) MEFs. c) Quantification of H4K8ac/DAPI in the main nuclei, nuclear blebs and micronuclei of Mof and Kansl2 KO (4 days); Nonparametric one-way ANOVA followed by a Kruskal-Wallis test. d) Representative immunostaining of K311R OST-lamin A MEFs for ATAC-see (white) and H3K27me3 (red). Arrows indicate nuclear abnormalities enriched for H3K27me3. e) Quantification of DAPI/dsDNA in the main nuclei and micronuclei of K311R lamin A MEFs; Two-tailed nonparametric Mann–Whitney test. f) Quantification of histone H3/DAPI in the indicated nuclear structures of Mof KO MEFs; Nonparametric one-way ANOVA followed by a Kruskal-Wallis test. g) Quantification of DAPI in the indicated nuclear structures of K311R lamin A MEFs; Nonparametric one-way ANOVA followed by a Kruskal-Wallis test. h) Representative immunostainings upon incubation with blocking peptides indicating specificity of the detected signal. Arrows indicate nuclear abnormalities. i) Quantification of bulk ATAC-see in MEFs expressing the indicated lamin A derivatives. Dots represent mean signal intensity. Nonparametric one-way ANOVA followed by a Kruskal-Wallis test. j) Immunoblotting on whole cell extracts of WT and K311R lamin A MEFs. Uncropped blots in Supplementary Fig. 9. k) Quantification of nuclear abnormalities on control and Mof KO (2 days) MEFs in the presence of the indicated GSK343 concentrations; Ordinary one-way ANOVA on n=3 independent biological replicates. l) Quantification of H3K27me3/DAPI in the main nuclei and nuclear abnormalities of control and Mof KO (2 days) MEFs in the presence of the indicated GSK343 concentrations; Nonparametric one-way ANOVA followed by a Kruskal-Wallis test. m) Quantification of Pol II-S2P in the main nuclei and nuclear abnormalities of control and Mof KO (2 days) MEFs in the presence of the indicated GSK343 concentrations; Nonparametric one-way ANOVA followed by a Kruskal-Wallis test. The data shown are the mean ± s.e.m. for a, c, e, f, g, k, l, m. dsDNA was counterstained using DAPI and scale bars of 5μm where applied to b, d, h. For a, c, e, f, g, l, m, n numbers are stated in the graphs and represent nuclear structures counted.