Extended Data Fig. 7: Reversibility of K-to-M dependent phenotypes.
(a) Histological analysis of testis in mice induced for 4 weeks with an additional 4 weeks of dox withdrawal. Scale bar = 50 μm. (b) Histological analysis of intestine in mice induced for 4 weeks with an additional 4 weeks of dox withdrawal. Black and white arrowheads indicate goblet cells and paneth cells, respectively. Scale bar = 50 μm. (c) Brightfield images of colonies in methylcellulose with dox removal. Black arrowheads indicate megakaryocytes. Scale bar = 200 μm or 50 μm. (d) Western blot analysis for tri-methyl H3K9 and H3K36 from EBs induced for 3 days with dox and EBs induced for 3 days with dox followed by 3 days of dox withdrawal. (e) Western blot analysis for H3K9me3 and H3K36me3 from HSPCs in mice induced for 7 days with dox and mice induced for 7 days with dox followed by 7 days of dox withdrawal. See source data for full membrane Western blot images. Data in a-e are representative of 3 independent experiments.
