Extended Data Fig. 8: K9I mice phenocopy K9M mice.
(a) Flow cytometry analysis of megakaryocyte progenitors (MkPs) cells, 4 weeks of dox. Frequencies as percentages of the parent gate. (b) Quantification of MkPs in bone marrow. Columns represent the mean +/− standard deviation (rtTA, n = 1; H3K9I, n = 3, biological replicates). (c) Platelet counts after 4 weeks of dox. Columns represent the mean +/− standard deviation (rtTA, n = 1; H3K9I, n = 3, biological replicates). (d) Flow cytometry of B cells in the bone marrow, 4 weeks of dox. Frequencies as percentages of the parent gates. (e) Quantification of mature B cells and IGM+ B cells. Columns represent the mean +/− standard deviation (rtTA, n = 1; H3K9I, n = 2, biological replicates). (f) Flow cytometry analysis of B cell progenitors in bone marrow, 4 weeks of dox. Frequencies as percentages of parent gates. (g) Quantification of ProB cells and PreB cells. Columns represent the mean +/− standard deviation (rtTA, n = 1; H3K9I, n = 2, biological replicates). (h) Flow cytometry analysis of LKS cells. Frequencies as percentages of parent gates. (i) Quantification of LKS cells in bone marrow. Columns represent the mean +/− standard deviation (rtTA, n = 1; H3K9I, n = 3, biological replicates). (j) Flow cytometry of stem cell populations gated on LKS cells. Frequencies indicated as percentages of parent gates. (k) Quantification of LMPPs, defined as Flt3high cells (top 25% of Flt3+ cells set on rtTA control). Columns represent means and error bars standard deviation (rtTA, n = 1; H3K9I, n = 3, biological replicates). (l) Flow cytometry of SLAM markers gated on LKS cells. Frequencies as percentages of parent gates. (m) Quantification of MPPs (left panel) and long-term HSCs (right panel) in bone marrow. Columns represent the mean +/− standard deviation (rtTA, n = 1; H3K9I, n = 3 biological replicates). Data in a,d,f,h,j,l are representative of 3 independent experiments.
