Extended Data Fig. 8: Chromatin association dynamics of CTCF, condensin, and cohesion.
From: A chromosome folding intermediate at the condensin-to-cohesin transition during telophase
a, Classification of cell cycle stages based on DAPI staining and tubulin organization. Scale bar = 5µm. b, Localization of Lamin A/C, NCAPH, and CTCF during different cell cycle stages shown in panel a. Scale bar = 5µm. c, Quantification of CTCF and NCAPH colocalization with chromatin in single cells at different cell cycle stages. Left plot represents data from all cells with color indicating cell cycle stage. Right plots represent the data separated into each individual cell cycle stage. d, Top: Western blot analysis of chromatin-associated proteins purified from HeLaS3-NCAPH-dTomato cells at different time points after release from prometaphase. Bottom: Quantification of the western blot shown above. NCAPH and Rad21 were analysed on the same gel. The samples for Histone H3 analysis were run on another gel. Four independent experiments were performed with similar results. Source Data for microscopy are provided in Source data Fig. 7. Unprocessed blots are provided in Source data Extended Data Fig. 8.
