Extended Data Fig. 7: CD9 expression in human PDAC.
From: CD9 identifies pancreatic cancer stem cells and modulates glutamine metabolism to fuel tumour growth
a, Co-IP of endogenous CD9 with endogenous ASCT2 after ASCT2 pull-down in the human pancreatic cancer cell line PANC-1. Input, left, 10% polyacrylamide gel; Co-IP, right, 20% polyacrylamide gel to separate bands from IgG heavy and light chains (asterisks). Blot representative of 2 independent experiments. See Source Data for uncropped blots. b, Co-immunofluorescence for CD9 and ASCT2 on human PDAC, showing punctate CD9 at the plasma membrane, with high levels of ASCT2 in CD9high cells; scale bars, 20 µm. Data are representative of 3 biologically independent human samples. c, Flow cytometry plots co-staining CD9 with other PDAC CSC cell surface proteins in primary human PDAC organoid culture. CD9 gate separates CD9high cells from bulk; gates on the x-axis separate marker-positive from marker-negative cells (based on fluorescence minus one controls). Plots are representative of 3 biologically independent primary organoid lines.
